INVESTIGADORES
BOSIO Gabriela Natalia
artículos
Título:
Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization
Autor/es:
BITZENHOFER, NORA LISA; HILGERS, FABIENNE; BOSIO, GABRIELA N.; TORRA, JOAQUIM; CASINI, GIORGIA; BEINLICH, FELIX R. M.; KNIEPS-GRÜNHAGEN, ESTHER; GORDELIY, VALENTIN; JAEGER, KARL-ERICH; NONELL, SANTI; KRAUSS, ULRICH; GENSCH, THOMAS; DREPPER, THOMAS
Revista:
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Editorial:
Humana Press Inc.
Referencias:
Año: 2022 vol. 2564 p. 143 - 183
ISSN:
1064-3745
Resumen:
Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
