TAXONOMÍA MORFOLÓGICA Y MOLECULAR DE ESPECIES DEL GÉNERO

BAROSSO ISMAEL; BIASOLI, MARISA; COLLETO, ANALIA; SCANDIANI, MARÍA M; LUQUE, ALICIA

Rosario

Congreso; Congreso XXVI Reunión Anual de la Sociedad de Biología de Rosario; 2006

Sociedad de Biología de Rosario

The Fusarium genus includes pathogens of plants, mycotoxigenics species and opportunists pathogens in humans and animáis. Conventional identification is carried out by colony morphology and conidial characteristics. The objective of the present study was to use PCR fingerprinting, to identifiy species of Fusarium. Eighty two Fusarium strains corresponding to 17 different species were used, 65 isolated as plant pathogens and 17 reference strains. The morphologic studies were carried out on PDA (pótalo dextrose agar) and SNA (synthetic nulrient lo agar) incubaled al 28°C. A minipreparation for DNA exlraction was used. In Ihe PCR fingerprinling a single primer, (GACA)4 was used. The amplification producís were separaled by eleclrophoresis in 1.4% agarose gels pholographied afler slaining with ethidium bromide. Of the 17 references of Fusarium strains used, 12 presented specific species profiles. The remaining 5 species, responsible for soybean sudden death syndrome (SDS) (of greal phylogenelic proximity), presented the sameprofíle thal allowed lo differentiate them from non SDS causing species. In most of the cases Ihe morphológica. Identifícalionscorresponded wilh the profiles of bands of the reference strain. This technique of PCR is a simple, rapid and sensilive assay, and has been of utility to confirm the phenotipic idenlificalion, specially in those species of Fusarium, thal by Iheir morphologic similarity, are difficult lo differentiate.