Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates

ENCINAS, M.; MARFIL, M.J.; GARBACCIO, S.; BARANDIARAN, S.; HUERTAS, P.; MORSELLA, C.; MACÍAS, A.; MAGNANO, G.; ZAPATA, L.; BIGI, F.; CATALDI, A.; PAOLICCHI, F.; ZUMÁRRAGA, M.; EIRIN, M.E.

Tuberculosis (Edinb)

CHURCHILL LIVINGSTONE

Año: 2018 vol. 111 p. 143 - 146

1472-9792

ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosisdiagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M.bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovisstrains exhibiting genomic variability (spoligotyping) were analyzed. Two genes ?esxA and esxB ? remainedinvariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in oneisolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotidepolymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv referencestrain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates andreinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should beperformed to globally confirm these findings.