INVESTIGADORES
EIRIN Maria Emilia
artículos
Título:
Human-T Lymphotropic Virus (HTLV) molecular diagnosis of indeterminate Western Blot cases in different populations in Argentina.
Autor/es:
BERINI, CAROLINA; MARIA EMILIA EIRIN; MARIA A. PANDO; MIRNA BIGLIONE
Revista:
JOURNAL OF MEDICAL VIROLOGY
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: Hoboken; Año: 2007 vol. 79 p. 69 - 73
ISSN:
0146-6615
Resumen:
Human T-cell lymphotropic virus (HTLV) seroindeterminate
cases have been reported among
blood donors (BD) and in at-risk populations
worldwide, including Argentina. The objective of
the present work was to study the presence of
HTLV-I/II infection and its association to specific
Western blot (WB) patterns among healthy BD
and at-risk populations in Argentina. We analyzed
83 HTLV-I/II seroindeterminate WB cases
diagnosed among BD (n¼49) and in different atrisk
populations (n¼34) for human retroviruses
infections. Multiple indeterminate WB patterns
were observed. Out of the total, 13.2% (11/83) of
the cases were found to be HTLV-I/II positive by
nested-PCR (n-PCR), including 13.2% (11/83)
HTLV-I and 2.4% (2/83) presenting HTLV-I and -II
co-infection. Most of their serological profiles
showed reactivity to gag or env codified proteins.
Two samples amplified only one of the six
analyzed genes (1 HTLV-I pol gene and 1 HTLV-II
tax gene). There was no association between the
presence of Trypanosoma cruzi infection and an
HTLV-I/II indeterminate WB pattern (only 3 of the
83 samples were positive for T. cruzi antibodies).
In conclusion, the majority of HTLV-seroindeterminateWBdonors
lacked HTLV provirus and was
thus considered uninfected. However, when
seroreactivity to Env and Gag proteins are
observed on the WB and especially in at-risk
populations, HTLV infection should be suspected;
such individuals should be followed-up
and retested.