Peroxiredoxins are Key Players of the Enzymatic Antioxidant System in Human Spermatozoa

MARÍA C. FERNÁNDEZ; ADEL R. MOAWAD; CRISTIAN O´FLAHERTY

Congreso; 31st Annual Meeting the protein society; 2017

Introduction and aim: Our lab has been pioneer to show that a family of antioxidant called peroxiredoxins (PRDXs) has a major role in the protection against oxidative stress in spermatozoa. However, how spermatozoa maintain PRDXs active remains unknown. Here, we aimed to determine the impact of different reactive oxygen species (ROS) levels produced by inhibiting specific players of the PRDX system on sperm viability and DNA oxidation. Experimental Approach: Highly motile spermatozoa from healthy donors were incubated with different inhibitors of the PRDX system and viability, ROS levels or DNA oxidation were asses by flow cytometry. Results: We observed a significant decrease in viable cells in a dose dependence manner when incubated with inhibitors of the 2-Cys PRDXs, calcium independent phospholipase A2 (PRDX6-PLA2) activity or PRDX re-activation system compared to controls (p