Inhibitory effect of lactobacilli on Candida albicans vaginal infection in an experimental mouse model.

PRISCILLA DE GREGORIO; MARIA CECILIA LECCESE TERRAF; MARIA SILVINA JUAREZ TOMAS; MARIA ELENA FÁTIMA NADER

Buenos Aires

Congreso; I Reunion conjunta de Sociedades de Biociencias- SAFE; 2017

11 Sociedades de Biociencias de Argentina

Vulvovaginal candidiasis (VVC) is one of the most frequent infection affecting the life quality of women worldwide, highly associated with Candida albicans (C.a.). Healthy vaginal microbiota is dominated by Lactobacillus, which form a defense line against pathogens. Beneficial vaginal lactobacilli were previously selected and evaluated in our laboratory. In this work, the effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 (CRL1324) or L. rhamnosus CRL1332 (CRL1332) against vaginal C.a. challenge in a murine experimental model was evaluated. Female BALB/c mice in pseudo-estrous were randomly assigned to five groups: a) Control: C.a. group [7 i.va. saline inoculations, later i.va. C.a. (106 CFU) challenge, and again 7 saline inoculations]; b) Preventive: CRL1324 or CRL1332 (7 doses) + C.a. group [7 i.va. CRL1324 or CRL 1332 (108 CFU) inoculations, C.a. challenge, and later 7 saline inoculations]; and c) Preventive-Therapeutic: CRL1324 or CRL1332 (7 doses) + C.a. + CRL1324 or CRL1332 (7 doses) group (7 i.va. CRL1324 or CRL 1332 inoculations, C.a. challenge, and 7 i.va. CRL1324 or CRL 1332 inoculations). Different studies were performed on days 1, 5 and 7 post-C.a. challenge: a) number of cultivable C.a., CRL1324 and CRL1332 from vaginal washing, and b) vaginal cytology and histology. Data were analyzed using ANOVA (general linear model). Fourteen doses of both CRL1324 and CRL 1332 were able to significantly reduce (P0.05). A significant increase (P