BEHAVIOR OF BENEFICIAL VAGINAL LACTIC ACID BACTERIA (BVLAB) TO THERMAL CONDITIONS FOR TECHNOLOGICAL APPLICATIONS

JESSICA SILVA; ANTONELLA MARCHESI; MARIA ELENA FATIMA NADER

Buenos Aires

Simposio; Reunion Conjunta de Sociedades de Bioiciencias; 2017

Sociedad Argentina de Farmacologia Experimental y 11 Sociedades Argentinas

Lactic Acid Bacteria constitute the main population of healthy vaginal microbiome, being responsible of the maintenance of urogenital tract physiological balance. Our research group have characterized BVLAB for the design of pharmaceuticals products, in which they need to maintain their viability and functional properties during industrial process and shelf life. Drying technological processes are applied, using high or drastic temperature changes that differ from the one required for their optimal growth, affecting the beneficial properties that support the selection of the probiotic strains. The aim of this work was to evaluate the BVALB survival to different high-temperature conditions. The strains studied were Lactobacillus reuteri (3 strains), L. gasseri (12), L. rhamnosus (4), L. salivarius (2), L. johnsonii (1), L. paracasei (2) and L. delbrueckii (6). BVLAB cells from the late-exponential growth phase were washed twice with buffer (PBS pH=6.5) and later inoculated (2%) in MRS broth, incubated at different temperatures (37ºC, 42ºC, 50ºC and 55ºC) for 24 hours. The growth was followed by optical density (OD560nm) at 3, 6, 9, 12 and 24 h to calculate growth´s parameters. CFU/ml was determined by plate culture method in some strains. Anova test was used for statistical evaluation. The results indicate that most of the strains showed similar growth patterns at 37 ºC and 42ºC with 95% significance (p