Effects of intramammary inoculation of Lactobacillus perolens CRL 1724 in lactanting cows´udders

IGNACIO FROLA; MATIAS PELLEGRINO; MARIA CAROLINA ESPECHE; JOSE GIRAUDO; MARIA ELENA FÁTIMA NADER; CRISTINA BOGNI

Journal of Dairy Research

CAMBRIDGE UNIV PRESS

Año: 2013 vol. 79 p. 84 - 92

0022-0299

Bovine mastitis, is one of the most expensive diseases in dairy farming. In a previous work, a strain of Lactobacillus perolens was isolated from milk of healthy dairy cows and characterized as potentially probiotic. In the present work we investigate the role of L. perolens CRL 1724 in inhibiting the indigenous microbiota bacteria (IMB) of the bovine teat canal and potential bovine mastitis pathogens(PBMP) as well as the in vitro capacity of this strain to adhere to bovine teat canal epithelial cells (BTCC). L. perolens was faced to fifteen ?colony types? indigenous microbiota microorganism and fourteen potential pathogen bacteria. The strain had an anti-microbial activity against 15 ?colony types?. Among them 13 ?colony types? (86.7%) showed an intermediate inhibition zone. A high percentage (71.4%) of inhibition zone against PBMP was observed. L. perolens CRL 1724 was able to co-aggregate most of thePBMP. An important pattern of adhesion of L. perolens CRL 1724 to BTCC (14.4 and 75% for the Adhesion index and Percentage of adhesion respectively) was observed. L. perolens CRL 1724 BTCC adhesion was confirm by Scanning Electron Microscopy. No morphologic or ultrastructural modifications of the cells referred to the adhesion of lactobacilli were found. The results obtained in this work provide enough evidence to considered L. perolens CRL 1724 as a potential probiotic strain to be included in the generation of non-antibiotic formulation for the treatment and prevention of mastitis in dairy cows.