Histological examination of non-lactating bovine udders inoculated with Lactobacillus perolens CRL 1724.

IGNACIO FROLA; MATIAS PELLEGRINO; GABRIEL MAGNANO; JOSE A GIRAUDO; MARIA CAROLINA ESPECHE; MARIA ELENA FÁTIMA NADER; CRISTINA BOGNI

Journal of Dairy Research

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2012 vol. 80 p. 28 - 36

0022-0299

ABSTRACTBovine mastitis produces a wide variety of problems in the dairy farm. The treatment of this disease isbased on the use of antibiotics which are not always effective. These drugs are also responsible for thepresence of residues in the milk and the increase of antibiotic-resistant strains. Probiotic products wereproposed as a valid alternative to antibiotic therapies and are also useful for the prevention of infectioussyndromes. With the aim of designing a probiotic product to prevent bovine mastitis, lactic acid bacteria(LAB) were isolated from foremilk samples from different dairy farms in Córdoba-Argentina. Onehundred and seventeen LAB were isolated and their beneficial characteristics such as the production ofinhibitory substances, surface properties and production of exopolysaccharides (EPS) were assessed.Most of them displayed low degree of hydrophobicity, autoaggregation, EPS negative phenotype andwere identified as Enterococcus hirae and Pediococcus pentosaceus. Nine LAB strains inhibited threeindicator bacteria. Some isolates were pre-selected and genetically identified according to the resultsobtained. Antibiotic resistance and virulence factors were studied for the assessment of the safety of thestrains. The results obtained were compared to those reported previously from samples obtained in theNorth-western area of the country and some differences were foun