Characterization of salivaricin CRL 1328, a two peptide bacteriocin produced by Lactobacillus salivarius CRL 1328 isolated from human vagina.

ESTEBAN VERA PINGITORE; MARIA ELVIRA HEBERT; MARIA ELENA FATIMA NADER; FERNANDO SESMA

RESEARCH IN MICROBIOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2009 vol. 160 p. 401 - 408

0923-2508

Salivaricin CRL 1328 is a heat-stable bacteriocin produced by LactobacillusLactobacillus salivarius CRL 1328, a strain isolated from healthy human vagina, with potentialsalivarius CRL 1328, a strain isolated from healthy human vagina, with potential applications for preventing urogenital infections. The objective of this study was toapplications for preventing urogenital infections. The objective of this study was to characterize the locus responsible for salivaricin CRL 1328 production and itscharacterize the locus responsible for salivaricin CRL 1328 production and its mechanism of action against Enterococcus faecalis MP97 as the sensitive strain.mechanism of action against Enterococcus faecalis MP97 as the sensitive strain. Oligonucleotides were designed based on sequences of antimicrobial peptidesOligonucleotides were designed based on sequences of antimicrobial peptides previously described in the literature. The salivaricin CRL 1328 cluster was identified,previously described in the literature. The salivaricin CRL 1328 cluster was identified, sequenced and analyzed. This cluster was similar to the previously described ABP118sequenced and analyzed. This cluster was similar to the previously described ABP118 which codified for a two-peptide bacteriocin. The putative mature peptides of salivaricinwhich codified for a two-peptide bacteriocin. The putative mature peptides of salivaricin CRL 1328, Salá and Salâ were chemically synthesized. These peptides did not showCRL 1328, Salá and Salâ were chemically synthesized. These peptides did not show bacteriocin activity when assayed individually. Both peptides exhibited optimalbacteriocin activity when assayed individually. Both peptides exhibited optimal antimicrobial activity at an equimolar ratio. Spectroscopic fluorescence assays wereantimicrobial activity at an equimolar ratio. Spectroscopic fluorescence assays were carried out using the synthetic peptides to study the effect of salivaricin on protoncarried out using the synthetic peptides to study the effect of salivaricin on proton motive force. This bacteriocin was shown to dissipate membrane potential and themotive force. This bacteriocin was shown to dissipate membrane potential and the transmembrane proton gradient, both components of proton motive force. E. faecalistransmembrane proton gradient, both components of proton motive force. E. faecalis MP97 cells treated with salivaricin CRL 1328 peptides were observed in transmissionMP97 cells treated with salivaricin CRL 1328 peptides were observed in transmission electron microscopy which revealed ultrastructural modifications of the cell wall.electron microscopy which revealed ultrastructural modifications of the cell wall.