Susceptibility of probiotic Lactobacilli to antimicrobial Agents

MARIA C OTERO; CLARA SILVA DE RUIZ; MARIA ELENA FÁTIMA NADER

Journal of Animal and Veterinary Advances

Medwell Journals

Año: 2007 vol. 6 p. 132 - 138

The aim of this work was the assessment of antibiotic susceptibility profile on bovine vaginal probiotic Lactobacillus. Antibiotics could affect the colonization of probiotic microorganisms when the host is exposed to therapies for other infections. We studied the sensibility of four strains of bovine probiotic vaginal Lactobacillus to ampicillin, vancomycin, gentamicin, kanamycin, lincomycin, tetracyclin, chloramphenicol, erythromycin, sulphamethoxazole, metronidazole, nitrofurantoin,  aminopenicillin/sulbactam and trimethoprim/sulfamethoxazole. The agar overlay disc diffusion and the dilution methods were performed according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The minimal inhibitory concentrations (MICs) obtained showed that L. gasseri CRL1412, L. gasseri CRL1421 and L. gasseri CRL1460 have similar susceptibility profiles. L. delbruekii subsp. delbrueckii CRL1461 was the most susceptible strain. The four strains showed high MIC values for the inhibitors of nucleic acid synthesis (>500 µg/mL for sulfamethoxazole and metronidazole), which are widely used in bovine therapies. None of the lactobacilli was resistant to vancomycin.These results are one of the studies required for the inclusion of these strains in a probiotic product for the reconstitution of the bovine urogenital microflora with Lactobacillus. Thus, can prevent metritis in cows during postpartum and increases the reproductive performance in dairy herd.