Involvement of microglia on the long -term consequences of early life stress in the visual system.

JUAN SALVADOR CALANNI; RUTH ROSENSTEIN; PASQUINI LAURA A

Mar del Plata

Congreso; SAIC; 2023

Early life stress (ELS) is defined as a period of severe and/or chronictrauma, as well as environmental/social deprivation or neglect inpre/postnatal stage. Presently, the impact of ELS on the visual systemin the adult stage is unknown. Using an animal model of maternalseparation with early weaning (MSEW), which mimics early lifeneglect, we analyzed the long-term ELS consequences in the visualsystem. Mice were separated from the dams for 2 h at postnataldays (PNDs) 2-5, for 3 h at PNDs 7-9, for 4 h at PNDs 10-13, for 6 hat PNDs 14-16, and weaned at PND17. At the end of each separationperiod, mothers were subjected to movement restriction for 10min. Control pups were left undisturbed from PND0, and weaned atPND21. At PND 60-75, MSEW did not affect the electroretinograma- and b-wave amplitude, but decreased retinal ganglion cell (RGC)function and number, and increased retinal Iba-1(+) area, and cellsoma size (by immunohistochemistry, P