INVESTIGADORES
MOREL Gustavo Ramon
congresos y reuniones científicas
Título:
Role of cooper and colesterol association in the neurodegenerative process.
Autor/es:
ARNAL N; MOREL GR; TACCONI DE ALANIZ MJ; DOMICINI L; MARRA CA
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVIII CONGRESO ANNUAL DE LA SOCIEDAD ARGENTINA DE NEUROCIENCIAS & Reunión satélite/ Neurobiología del comportamiento y Neurobiología de la memoria en el cono sur.; 2013
Institución organizadora:
SAN
Resumen:
Age is one of the main factors involved in the development of neurological illnesses, in particular, Alzheimer, and it is widely held
that the rapid aging of theworld population is accompanied by a rise in the prevalence and incidence ofAlzheimer disease.However,
evidence fromrecent decades indicates that Cu and Cho overload are emerging causative factors in neurodegeneration, a hypothesis
that has been partially investigated in experimental models. The link between these two variables and the onset of Alzheimer
disease has opened up interesting new possibilities requiring more in-depth analysis. The aim of the present study was therefore to
investigate the effect of the association of Cu + Cho (CuCho) as a possible synergistic factor in the development of an Alzheimer-like
pathology inWistar rats.We measured total- and nonceruloplasmin-bound Cu and Cho (free and sterified) contents in plasma and
brain zones (cortex and hippocampus), markers of oxidative stress damage, inflammation, and programmed cell death (caspase-3
and calpain isoforms). The ratio beta-amyloid (1-42)/(1-40) was determined in plasma and brain as neurodegenerative biomarker.
An evaluation of visuospatial memory (Barnes maze test) was also performed. The results demonstrate the establishment of a
prooxidative and proinflammatory environment after CuCho treatment, hallmarked by increased TBARS, protein carbonyls, and
nitrite plus nitrate levels in plasma and brain zones (cortex and hippocampus) with a consequent increase in the activity of calpains
and no significant changes in caspase-3. A simultaneous increase in the plasma A𝛽1-42/A𝛽1-40 ratio was found. Furthermore, a
slight but noticeable change in visuospatial memory was observed in rats treated with CuCho.We conclude that our model could
reflect an initial stage of neurodegeneration in which Cu and Cho interact with one another to exacerbate neurological damage.