Genetic fingerprint and diversity evaluation of halophilic Bacillus species by RAPD-PCR

ORCE, INGRID GEORGINA; MARTÍNEZ, FABIANA LILIAN; APARICIO, MÓNICA; TORRES, MARÍA JULIA; RAJAL, VERÓNICA BEATRIZ; IRAZUSTA VERÓNICA

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS.

ACAD BRASILEIRA DE CIENCIAS

Año: 2021

0001-3765

Random amplified polymorphic DNA-PCR (RAPD-PCR) is a technique successfully used to generate characteristic fingerprints of different bacteria. Bacillus is a genus that includes heterogeneous species, thus a combination of different techniques is essential for their identification. Here we used RAPD-PCR methodology to distinguish among genetically similar strains and to evaluate the genetic diversity of Bacillus species from the Salar del Hombre Muerto, in the Northwest of Argentina. The RAPD-PCR used allowed obtaining different amplification profiles for each Bacillus species and strains. By comparing fingerprint profiles, the RAPD-PCR allowed identifying some of the salt flat isolates. As expected, the bacilli group isolated revealed a wide heterogeneity. RAPD-PCR was found to be a quick and reliable technique to evaluate the diversity of Bacillus strain and was successfully applied to characterize the genetic diversity present in the Salar del Hombre Muerto.