CARBOXYLATED LATEX PARTICLES SENSITIZED WITH ANTIGENIC PROTEINS OF VARIOUS DISEASES FOR THEIR USE AS IMMUNOAGGLUTINATION REAGENTS

GONZALEZ, VERÓNICA DG.; GARCÍA, VALERIA; PERETTI, LEANDRO E; PROCHETTO, SANTIAGO; GUGLIOTTA, LUIS M.

Florianópolis

Congreso; 12º Congresso Brasileiro de Polímeros; 2013

Associação Brasileira de Polímeros

Monodisperse polymer colloids with carboxyl functionality were synthesized through a two-step emulsion polymerization process. The synthesized ?core-shell? particles were used as support for their sensitization (by covalent coupling) with antigens of Trypanosoma cruzi, Toxoplasmas gondii and Leptospira interrogans. The resulting latex-protein complexes were used in immunoagglutination assays for detecting Chagas disease, Toxoplasmosis and Leptospirosis, respectively. In all cases, high fractions of proteins chemically-bound to carboxyl groups were observed and all latex-protein complex allowed discrimination between sera from infected and non-infected patients.