Synthesis and Characterization of Latex-protein Complexes from Different Antigens of Toxoplasma gondii for Immunoagglutination Assays

PERETTI, LEANDRO E; GONZALEZ, VERÓNICA DG.; COSTA, JUAN G; MARCIPAR, IVÁN S.; GUGLIOTTA, LUIS M.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS

TAYLOR & FRANCIS AS

Lugar: Londres; Año: 2016 vol. 65 p. 938 - 946

0091-4037

The acute phase recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite were used to produce latex-protein complexes (LPC) through physical adsorption (PA) and chemical coupling (CC) onto different latexes, with the aim of producing immunoagglutination (IA) reagents able to detect recently acquired Toxoplasmosis. Polystyrene and ?core-shell? latexes where employed, exhibiting varied particle size, functionality (carboxyl or epoxy) and charge density. The synthesized LPC were employed in IA assays. To this effect, the agglutination reaction was followed by measuring the changes in the optical absorbance by turbidimetry. Experiments against control sera were performed to evaluate the performance of various LPC and it was observed that the IA test based on P22Ag and the carboxylated latex of 350 nm of particle diameter allowed a good discrimination between acute sera and chronic/negative ones. The proposed test is cheap, rapid and easy to implement.