In vivo curative activity of enological yeasts against Botrytis cinerea in grape

MUÑOZ M.A.; NALLY M.C; PESCE V.M.; RADICETTI D.S.; TORO M.E.; VAZQUEZ F.

San Juan

Otro; 2da Reunión Conjunta de las Sociedades de Biología de la República Argentina; 2011

Yeasts have been studied because they possess many features which make them suitable as biocontrol agents in fruits. These microorganisms show a protective effect that diminishes with fruit ripening and senescence (preventive effect), and there are few reports about their curative activity (eradicant effect). The aim of the present work was to evaluate the in vivo curative activity of enological yeasts against B.cinerea in Superior Grape. The curative activity assay was performed using 40 wine yeasts, which had antagonistic activity in vitro and in vivo preventive effect assays; against B.cinerea B14. Spores suspension (20μL) of B.cinerea (104spore/ml) was inoculated into a wound. After 2 and 24h 20μL of yeasts (106cfu/ml) were inoculated. Fruits were stored at 20°C for 7 days. Incidence was determined. After 2h of yeasts inoculation, 17 isolates B.cinerea inhibition (8 S.cerevisiae, 1 S.kluyveri, 1 S.bayanus, 4 T.delbrueckii, 1 C.sake, 1 D.vanrijiae, 1 D.hansenii). After 24h of yeasts inoculation, 28 isolates showed antagonistic activity (17 S.cerevisiae, 5 T.delbrueckii, 1 C.sake, 1 S.bayanus, 1 S.chevalieri, 1 S.kluyveri, 1 H.uvarum, 1 D.vanrijiae). These enological yeasts have curative activity against B. cinerea. This work represents an initial step for further research in other pathosystems in which B.cinerea is a serious pathogen.