Effects of bacterial lipopolysaccharide and Shiga Toxin on induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells

MARTIRE-GRECO, DAIANA; LA GRECA, ALEJANDRO; CASTILLO LUIS ALEJANDRO; CELESTE BIANI; LOMBARDI ANTONELA; BIRNBERG-WEISS, FEDERICO; NORRIS ALESSANDRA; SACERDOTI FLAVIA; AMARAL MARTA; RODRIGUEZ-RODRIGUES NAHUEL; PITTALUGA, JOSE R.; FURMENTO VERONICA; LANDONI, VERÓNICA I.; MIRIUKA, SANTIAGO G.; LUZZANI, CARLOS; FERNÁNDEZ GABRIELA FERNANDEZ

SHOCK Injury, inflamation and Sepsis: Laboratory and Clinical Approaches

Wolters Kluwer

Año: 2023 vol. 59 p. 941 - 947

Background: Mesenchymal Stem Cells can be activated and respond to different bacterial toxins. Lipopolysaccharides (LPS) and Shiga Toxin (Stx) are the two main bacterial toxins present in Hemolytic Uremic Syndrome (HUS) that cause endothelial damage. In this work we aimed to study the response of iPSCMSC to LPS and/or Stx and its effect on the restoration of injured endothelial cells.Methods: iPSC-MSC were used as a source of mesenchymal stem cells (MSC) and Human Microvascular Endothelial Cells-1 (HMEC-1) as a source of endothelial cells. iPSC-MSC were treated or not with LPS and or/Stx. For some experiments, Conditioned Media (CM) were collected from each plate and incubated with an anti-Stx antibody to block the direct effect of Stx, or Polymyxin to block the direct effect of LPS. In CM from both treatments, anti-Stx and Polymyxin were used. Results are expressed as mean ± S.E.M. Significant differences (p