Pathogenicity, formulation and storage of insect pathogenic hyphomycetous fungi tested against Diabrotica speciosa

V.F.CONSOLO, G.L.SALERNO AND CM BERÓN

BIOCONTROL (DORDRECHT)

Kluwer Academic Publishers

Lugar: The Netherlands; Año: 2003 vol. 48 p. 705 - 712

1386-6141

Studies were conducted to search for fungal strains with potential pathogenicity against Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae). Among sixteen fungal isolates screened the most virulent was a Beauveria bassiana (Balsamo) Vuillemin isolate (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of isolates screened the most virulent was a Beauveria bassiana (Balsamo) Vuillemin isolate (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of Diabrotica speciosa (Germar) (Coleoptera: Chrysomelidae). Among sixteen fungal isolates screened the most virulent was a Beauveria bassiana (Balsamo) Vuillemin isolate (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value of Beauveria bassiana (Balsamo) Vuillemin isolate (FHD13) that caused 70% mortality of D. speciosa third instar larvae. The LC50 value ofD. speciosa third instar larvae. The LC50 value of B. bassiana isolate FHD13 was 3.48 × 1010 conidia/ml. Different temperatures (4, 17 and 26 .C) and vegetable oils (corn, sunflower and canola) used for storage did not significantly affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. 26 .C) and vegetable oils (corn, sunflower and canola) used for storage did not significantly affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. isolate FHD13 was 3.48 × 1010 conidia/ml. Different temperatures (4, 17 and 26 .C) and vegetable oils (corn, sunflower and canola) used for storage did not significantly affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. .C) and vegetable oils (corn, sunflower and canola) used for storage did not significantly affect viability of conidia. A pathogenicity trial against D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality. D. speciosa larvae performed with the corn oil formulation (1 × 108 conidia/ml of oil) caused 65% of mortality.× 108 conidia/ml of oil) caused 65% of mortality.