Analytical eco scale in the extraction of phenolic compounds mediated by natural solvents

ESPINO MAGDALENA BELEN; FERNÁNDEZ MARÍA DE LOS ANGELES; GOMEZ, FEDERICO J.V.; SILVA MARIA FERNANDA

Morelia, Michoacan

Simposio; 23rd Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2017

Green Analytical Chemistry is based on the design and development of analytical proceduresthat minimize the amount of hazardous substances used and generated [1,2].The analytical Eco-Scale proposed by Galuzka et al. [3] consists in assigning penalty points (PPS) to parametersof an analytical process that are not in agreement with the ideal green analysis.The extractionis a key step since the main analytical techniques use high volumes of organic solvents of lowbiodegradability, high cost and high environmental impact. In this context, one of the greatestchallenges that Green Chemistry faces is the development of sustainable solvents. Natural DeepEutectic Solvents (NADES) are introduced as a new innovative class of green extraction media.These sustainable solvents are constituted by a mixture of compounds naturally present in thecell metabolism such as sugars, organic acids, urea and choline chloride. Their use in theextraction step would contribute to the development of sustainable analytical procedures.Bioactive compounds from medicinal plants have been widely studied, since they are attributedwith important antimicrobial, anti-inflammatory and antioxidant properties, among others. Inthe present work, an extraction procedure mediated by a NADES was applied for the recoveryof phenolic compounds from Larrea divaricata, an Argentinian autochthonous plant. Theextract was analysed by HPLC-DAD, with naringenin, caffeic and ferulic acid, tyrosol andnordihydroguaiaretic acid detected. Analytical Eco-Scale for assessing the greenness of thedeveloped procedure was calculated and compared with traditional extraction techniques. Theresults highlighted that methodology mediated by NADES is in agreement with green analysis,reaching a 75% reduction of PPS with high phenolic compounds extraction efficiency.