INFERRING RECOMBINATION EVENTS IN PLANT MITOCHONDRIA USING HIGH-THROUGHPUT SEQUENCING DATA

GANDINI, CAROLINA L.; GARCIA, LAURA E.; ABBONA, CINTHIA C.; SANCHEZ-PUERTA, M. VIRGINIA

Mendoza

Congreso; A2B2C 10th Meeting; 2019

Sociedad Argentina de bioinformática y Biología computacional

Angiosperm mitochondrial genomes (mtDNA) exhibit extremely high rate of recombination and sequence rearrangement. Moreover, the ability to incorporate foreign sequences into their genomes is though to be mediated by homologous recombination(HR). An interesting way to study recombination in plant mtDNA consist in the generation of somatic hybrids. Our lab has recently studied two somatic hibrids between the solanaceae Hyoscymus niger and Nicotiana tabacum. The mtDNA was highly chimeric and ocurred entirely by HR mechanisms, most by the break induced replication (BIR) pathway. In this study we produced somatic hybrid between the Solanaceae Physoclaina orientalis and N. tabacum and we analyzed the recombination map of a somatic hybrid. To do so, we performed high -throuput sequencing ans developed a novel bioinformaic strategy that infers recombination events by identifying parental genomic switches based on paired- end reads.pre.cjk { font-family: "Courier New", monospace }p { margin-bottom: 0.1in; line-height: 115% }