EFECTO DE LOS MICRONUTRIENTES SOBRE LA CANTIDAD DE LÍPIDOS EN LA PRÓSTATA DE RATAS

FERRAMOLA, M, LUCERO, R; BIAGGIO, V; GATICA, L; GIMENEZ, MS

Uspallata - Mendoza

Otro; XXIIIREUNION ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO – III REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE MICROSCOPIA; 2005

Universidad Nacional de Cuyo

ABSTRACT Micronutrients are essential in the diet, in order to maintain cellular integrity. We previously reported that Zn deficiency modifies phospholipids and cholesterol concentration in lung and epididymis. In the same manner, deficiency of vitamin A (vit A)modifies the amount of these lipids in aorta, heart and liver. Here we present the results of phospholipids and cholesterol measurements in rat prostates. Rats were separated in six groups: I- control Zn (+), IIZn (-), III- Zn (-) refed with Zn (+), IV- control vit A (+), V- vit A (- ), IV- vit A (-) refed with vit A (+). Groups I, II and III were fed during 2 months; groups IV, V, VI, were fed during 3 months. In the case of groups III and VI, they were refed during two weeks with the respective control diet. Afterwards rats were killed under light ether anesthesia. Their prostates were removed, weighted and kept at -70ϒ C until processed. The lipids were separated by Folch method, cholesterol was measured according to Zak et al, and phospholipids as described by Bartlett et al. The results show that these micronutrients did not change phospholipids and cholesterol concentrations in prostate. Thus, Zn and vitamin A deficiencies selectively affect lipid concentration in different rat organs.