EFFECT OF ANDROGEN DEPRIVATION ON LUNG STROMAL INTEGRITY OF MALE RAT

PIGUILLEM SN; BOLDRINI G; PEREZ CHACA MV; ALVAREZ SM; BIAGGIO VS; CIMINARI E; GOMEZ NN

Congreso; Sociedad Argentina de Investigacion Clinica; 2017

ociedad Argentina de Investigacion Clinica

Even though androgens are fundamental in lung function otherwise the castration is the standard therapeutic treatment for advanced prostate cancer. Additionally, epithelial cells produce a big amount of transforming growth factor-β1 (TGF-β1) and inflammatory cells during lung injury. TGFβ1 plays a crucial role in differentiation, proliferation, and apoptosis process, including apoptosis of airway epithelial cells. Objetive: to explore epithelium integrity in the course of androgen deprivation. Male Wistar rats (200 ± 20 g) were separated in 3 groups: controls (Co), castrated (Ca), and castrated supplemented with testosterone (Ca+T) for five days. After 60 days rats were killed and lungs were obtained. RNA was extracted by the method of TRIzol. mRNA levels of Bax, Bcl-2, cyclooxygenase-2 (COX-2), vascular cell adhesion protein-1 (VCAM-1), TGFβ, androgen receptor (AR) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were quantified by RT-PCR, using s28 as control. TBAR?S and testosterone level were measured. Lungs were examined for evidence of injury. ANOVA was used for statistical analysis. Testosterone decrease (p