DETERMINATION OF CADMIUM-INDUCED INFLAMMATORY PARAMETERS IN WISTAR RAT CEREBRAL CORTEX

BIAGGIO VS; RUSSO MG; PEREZ DIAZ, M; SANTILLAN L ; ANTÓN R; GIMENEZ MS

Mar del Plata

Congreso; SOCIEDAD ARGENTINA DE BIOQUIMICA Y BIOLOGIA MOLECULAR (SAIB); 2009

SOCIEDAD ARGENTINA DE BIOQUIMICA Y BIOLOGIA MOLECULAR (SAIB)

It has been observed that cadmium causes changes in the level of expression of genes involved in inflammatory disorders in different types cell in cerebral cortex. In this work we measured the concentration of cadmium in samples of cerebral cortex, the expression of inflammatory (TNFa,VCAM1 and iNOS) antiinflammatory parameters (PPARg and MT III) and parameters oxidative stress (TBARS and NO). Our experimental model consisted of adult-male Wistar rats subjected to 15 ppm of cadmium in drinking water (Cd group) and without it (group CO). The total RNA was extracted by the method of TRIzol and levels of RNAm quantified using RT-PCR, the TBARS were quantified and the levels of NO using the reaction of Gries. We observed a significant increase in the concentration of cadmium in cerebral cortex (p