INVESTIGADORES
BIAGGIO Veronica Silvina
congresos y reuniones científicas
Título:
EFECTO DE LA DEFICIENCIA DE ZINC EN LA EXPRESIÓN DE PARÁMETROS DE INJURIA PULMONAR
Autor/es:
BIAGGIO VS; ALVAREZ SM; GATICA LV; GIMENEZ MS; GOMEZ NN
Lugar:
Misiones - Iguazú
Reunión:
Congreso; SOCIEDAD ARGENTINA DE INVESTIGACION CIENTÍFICA y BIOLÓGICA (SAIB); 2004
Institución organizadora:
Sociedad Argentina de Investigación Cientifica yBiológica (SAIB)
Resumen:
Zinc is a micronutrient whose deficiency is associated with
oxidative stress. Besides, nitric oxide (NO) plays an important
role in the pulmonary physiology, but the expression of the
inducible isoform of the enzyme that synthesizes NO (iNOS) is
presented in lung injury. It is known that PPARγ and TGF-β1 are
factors whose expression is related to inflammatory situations.
To evaluate the effect of lung injury in the chronic Zinc deficiency
we studied the expression of iNOS, PPARγ and TGF-β1 in rat
lung. Wistar male rats (200 ± 20 g) were fed with AIN-93 diet
(zinc deficient, ZD) or with 30 mg Zn/ kg (Control, Co) during 2
months. Total RNA was isolated from lung by using TRIzol. 1 μg
of RNA was transcribed to cDNA at 42°C using random hexamers
as primers and RT-MMLV (Moloney Murine Leukemia Virus).
Aliquots of 2 μl of cDNA were used in the amplifications by PCR
using specific primers for the following genes: iNOS and PPARγ.
Beta actin was used as an internal control. 100 mg of lung were
homogenized in the presence of protease inhibitors and 40 μg of
proteins were separated in an 10% SDS-PAGE, transferred to
PVDF membranes and incubated with an antibody against TGF-
β1. The bands were quantified by densitometry.
We detected a significant increase in iNOS (p< 0.001) and TGF-
β1 (p