VARIACIONES EN LA EXPRESIÓN DE MARCADORES PRO-INFLAMATORIOS EN MACRÓFAGOS ALVEOLARES DESPUÉS DE LA CASTRACIÓN.

BIAGGIO VS; PIGUILLEM S; PEREZ CHACA MV; CIMINARI E; ALVAREZ SM; GOMEZ NN

Congreso; SOCIEDAD DE BIOLOGIA DE CUYO; 2014

Previously, we demonstrated an increased expression of several pro-inflammatory parameters in lung parenchyma at 30 and 60 days after castration. Alveolar macrophages (AM) are derived from monocyte precursor under tissue specific differentiation and infiltrate the site of infection or injury to produce inflammatory mediators including chemokines, cytokines and other products of proteolytic cascades. Nrf2/ARE pathway is critical for the regulation of intracellular redox status. TNF-α is a pro-inflammatory cytokine that promotes endothelial dysfunction. We decided to study how the time of castration (30 and 60 days) affected on the expression of these markers of inflammation and oxidative stress. Male Wistar rats (200 ± 20 g) strains were separated in 3 lots: controls (Co), castrated (Ca), and the group of castrated rats to which were administered testosterone for five days (Ca + T) then castration. After 30 and 60 days we proceeded to the extraction of the lungs. Bronchoalveolar lavage (BAL) was obtained and the cell count was performed. RNA was extracted by the method of TRIzol. The levels of RNAm were quantified using RT-PCR and oxidative stress biomarkers were measured. ANOVA was used for statistical analysis. The percentage of cells count in BAL at 30 and 60 days, showed an increased of neutrophils (23%) and lymphocytes (25%) in Ca group compared to Co. At 30 days the expression of Nrf-2 and Nox decreased Ca group compared to Co (p