EFFECT OF A HYPERCALORIC DIET ON HYPOANDROGYNOUS RAT LUNG

BIAGGIO VS; ABALLAY FEDERICO; SALINAS M; ZELARAYAN SARMIENTO D; PIGUILLEM SN; CIMINARI E; RAZZETTO G; SALINAS ELOY; PEREZ CHACA MV; ALVAREZ SM

Mar del Plata

Congreso; SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA; 2022

Sociedad Argentina de Investigación Clínica

Obesity isa systemic state of inflammation and oxidative stress that affects normalrespiratory functioning. The presence of androgen receptors in lung reveal thatthese hormones might play a key role in lung physiology. Obesity-mediatedoxidative stress produced in adipose tissue is one of the main factorsconsidered as oxidant source and inflammation mediator. The aim of this workwas to study the effect of diet-induced obesity on the lung ofandrogen-deficient rats (castrated). Wistar male rats (200 ± 20 g) wereseparated in four groups: Control with normal diet (CoN), castrated with normaldiet (KN), control with hypercaloric diet (CoOB) and castrated withhypercaloric diet (KOB) and sacrificed 30 days after castration. Biochemicalparameters were analysed in serum and the expression of antioxidant enzymes andNOX-2, FOXO, HO-1 and RA in lung. ANOVA and Tukey test were used forstatistical analyses. The results showed TBARS levels increased in KOB groupcompared to CoN (p<0.001) and KN (p<0, 01) groups, respectively. CATactivity was increased in KN (p<0.05) group. HDL levels increased in CoOB(p<0.001), KN (p<0.001) and KOB (p<0.01) groups. Both, urea and TGdeterminations were increased in KN (p<0.001); p<0.01 and KOB(p<0.001) groups. CL was increased in CoOB (p<0.001); KOB (p<0.01)compared to CoN group and increased in KN (p<0.001) and KOB (p<0.001)compared to CoOB group. CAT expression decreased in CoOB and KN groups, and RAexpression increased in KN group compared to control group. Antioxidant enzymesNOX-2 and SOD-2 (p<0.01) and GPx-1(p<0,05) increased in KOB groupcompared to control. FOXO-1 and HO-1increased in castrated group, withobesogenic diet (p<0.01). We previously demonstrated an important oxidativestress state in a castrated animal model. In conclusion, obesity added toandrogen deficiency modifies different serum parameters. In fact, someinflammatory molecular pathways reveal a potential relationship between bothsituations (androgen deficiency-obesity).