Overexpression of inducible nitric oxide synthase and ciclooxigenase 2 in rat zinc-deficient lung: Involvement of NF-kB dependent pathway

GOMEZ. NIDIA; DAVICINO, ROBERTO; BIAGGIO, VERÓNICA; BIANCO, GERMÁN; ALVAREZ, SILVINA; FISCHER, PATRICIA; MASNATTA, LUCAS; RABINOVICH, GABRIEL; GIMENEZ, MARÍA SOFÍA

NITRIC OXIDE-BIOLOGY AND CHEMISTRY

Elsevier

Lugar: Inglaterra; Año: 2005 vol. 14 p. 30 - 38

1089-8603

Abstract Nitric Oxide 14 (2006) 30–38 TITULO. Overexpression of inducible nitric oxide synthase and cyclooxygenase-2 in rat zinc-deficient lung: Involvement of a NF-_B dependent pathway AUTORES: Nidia N. Gomez , Roberto C. Davicino , Veronica S. Biaggio , German A. Bianco ,Silvina M. Alvarez , Patricia Fischer , Lucas Masnatta, Gabriel A. Rabinovich María S. Gimenez Abstract Reactive oxygen and nitrogen species have been implicated in the pathogenesis of pulmonary diseases. The goal of this study was to measure the response of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 enzymes (COX-2) in lung with moderate zinc deficiency. Adult male Wistar rats were divided into two groups receiving (1) a zinc-deficient diet (ZD) or (2) a zinc-adequate control diet. After 2 months of treatment, the zinc-deficient group showed a significant pulmonary edema. This was associated to a reduction of protein thiols and to a significant increase of metallothionein and glutathione disulfide levels. In addition, a higher serum and lung NO production in ZD group was positively related to the higher activity and expression of iNOS and COX-2 found in lungs. Western blot analysis revealed increased IkBa degradation, an indicator of NF-_B activation in ZD lungs. Anatomopathologic analysis of ZD lungs showed an increase of connective tissue fibers with an influx of polymorphonuclear cells. These cells and type II cells from the alveoli showed specific immunohistochemical signals for iNOS. The conclusion is that, during the development of zinc-deficiency, iNOS activity increases in lung and contributes to lung injury. Zinc deficiency implications must be taken into account to design therapies and public health interventions involving targeted zinc supplementation for high-risk subjects or certain diseases, such as asthma. Keywords: Lung; Zinc-deficiency; iNOS; COX-2; NF-kB