Androgen Deprivation and Cytoprotective Parameters in Rat Lung

SILVINA M ÁLVAREZ; VERÓNICA S BIAGGIO; MARÍA E CIMINARI; SILVANA N PIGUILLEM; ANAHÍ B PACHECO GUIÑAZÚ; SOPHIA M ESCOBAR CORREAS; MARÍA V PEREZ CHACA; NIDIA N GOMEZ

International Journal of Research Studies in Biosciences (IJRSB)

Balaji- India

Año: 2015 vol. 3 p. 150 - 163

2319-2844

AIM: To study the effect of androgen deprivation, focusing on oxidative stress parameters, apoptosis and citoprotective markers, in order to better understand the possible causes of pulmonary diseases in elder people that normally undergoes a decrease or eventual deprivation of testosterone. MATERIALS AND METHODS: Adult male Wistar rats weighing 200 ± 20 g were divided into three groups: Group I that was used as control (Co), group II was subjected to castration by simple orchidectomy (Ca) and group III was subjected to orchidectomy and received testosterone (100 µg/kg body weight) daily from day 25 to day 30 after castration, by intramuscular injection (im) (Ca + T). Bronchoalveolar lavages (BAL), serum, and lung were obtained. We determined TBARS, nitrite and proteins content in BAL and lung. We also performed RT-PCR in lung in order to analyze the expression of nrf-2 factor ,NOX, SOD, GPx and AR. The expression of Hsp 70i and Hsp 27 was analyzed by immunohistochemistry. The statistical analyses were done by ANOVA (GraphPad Prism 5.0, software). After that, individual comparisons were made with the Dunnett´s multiple-comparison test. Differences between means were considered significant at the p< 0.05 level. RESULTS: We observed a decrease in body weight in the castrated groups. Protein content in (BAL) was increased in both castrated groups (p