"Modelo de carcinogénesis cutanea en ratones hairless (SKH: HR1) por irradiación UV-B. Probable rol de la apoptosis en la inducción de lesiones".

SQUIQUERA L.; GONZALEZ MAGLIO D.; GELMI L.; PAZ M.L.; FRIDMANIS M.; BUSTAMANTE J.; LEONI J.

Revista Argentina de Dermatología

Año: 2001 vol. 82 p. 218 - 227

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Apoptosis or programmed cell death is a complex mechanism by which cells that have suffered genetic damage are eliminated. We have developed a cancer model induced byUVBin Hairless mice. In our model, the animals developed lesions with histologic diagnosis of squamous cell carcinoma after 4 months with suberitematogenous doses of UVB irradiation. The early effects of radiation was evaluated by irradiating the animals with a minimal erytematogenous dose. Skin biopsies were obtained from the dorsal skin and processed for routine H&E staining, and apoptosis (TUNEL). Seventy two hours post irradiation, a maximal tissular response consisting on hyperplasia and dysplasia such as suprabasal mitosis and loss of architecture. The TUNEL staining revealed scarce apoptotic cells in the epidermis and intense apoptosis in the dermal components such as mononuclear cells and fibroblast.