Abnormal/dysfunctional dendritic cell generation from bone marrow of lipopolysaccharide-immunosuppressed mice.

LANDONI V.I; RODRIGUEZ-RODRIGUES N; ; CHIARELLA, P; CASTILLO, L; MARTIRE-GRECO, D; ; SCHIERLOH LP;; ISTURIZ, MA.; FERNÁNDEZ, GC

Mar del plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2016

An immunosuppressed state is elicited in some patient at late phases of sepsis, evidenced by a reduced T cell response and antibody production. A loss of dendritic cells (DC) in blood and lymph nodes (LN) has been also described in septic patients. Considering this, we hypothesized that alterations in the number/function of DC may contribute to the immunosuppression (IS) associated to sepsis. Repetitive, increasing doses of LPS in mice mimics this state of IS. The aim of this work was to evaluate the phenotype and functional state of DC from IS mice. We found that the number of DC (CD11c+) in LN of IS mice was lower compare to Control (Ctrol). Then we traced DC migration to LN by the FITC skin painting method. We found a reduced number of DC (CD11c+FITC+) mobilized to LN in IS mice (p