Latex colored particles: synthesis and BSA absorption for potential applications in immunochromatography

FEMIA, L; GONZÁLEZ, V.D.G.; MINARI, R.; ALONSO, S. DEL V.; GUGLIOTTA, L.

Carlos Paz, Córdoba

Congreso; XLII Reunión Anual de la Sociedad Argentina de Biofísica; 2013

Sociedad Argentina de Biofísica

Colored latexes of polystyrene have acquired much interest in biological fields, since they are applicable in many topics [1]. Several authors had reported the synthesis of these materials, and its interaction with proteins [2]. There are several potential applications like immunochromatography, immunoagglutination assays, particle designs for flux cytometry, protein-latex detection kits, etc. In view of the increasing interest in the use of these particles, we have synthesized a new polymerizable colorant from basic fuchsine (BF). The functionalized colorant was characterized by NMR, FTIR and ESI-mass spectrometry. Latex colored particles were prepared with this color-monomer and with the non-functionalized colorant, BF. The obtained particles were characterized in terms of size, color incorporation, conversion efficiency in the synthesis process and protein adsorption (BSA). We have obtained purple colored latex particles with a sized range from 80-110 nm, which were stable in the presence of emulsifier. The monomer conversion was higher in the styrene/colored-monomer reaction than styrene/BF reaction. Color incorporation was not significantly different but, color leakage was greater for polystyrene/BF system.