MOLECULAR EPIDEMIOLOGY OF HPV IN HEALTHY SKIN FROM IMMUNOSUPPRESSED AND IMMUNOCOMPETENT INDIVIDUALS

SANCHEZ, ADRIANA; FERNANDEZ BUSSY (H), RAMON; NIPOTI, JESICA; PICCIRILI, GUSTAVO; BOLATTI, ELISA; CHOUHY, DIEGO; CASAL, PABLO; STELLA, EMMA; GIRI, ADRIANA; FERNANDEZ BUSSY RAMON A

Vancouver

Congreso; 23rd World Congress of Dermatology; 2015

Abstract: Papillomaviruses (PVs) are DNA tumour viruses infecting mucosal and cutaneous epithelia of vertebrates. More than 190 human PVs (HPVs) types have been completely sequenced. Although the clinical implications of thecutaneous HPV types are still unknown, it has been proposed that immunosuppression (UV + immunosuppressive treatment) may contribute to HPV infection. In order to explore the diversity of cutaneous HPVs on healthy skin fromindividuals with different immunological status, we designed a transversal descriptive study including 100 immunocompetent (IC) and 64 immunosuppressed (IS) individuals. Skin samples were collected from the forehead using a noninvasive procedure and analyzed by "hanging droplet" PCR with the FAP primer system. HPV DNA was more frequently found in the IC group (65% IC vs. 56% IS). In both populations, the majority of HPV types and putative types identified belonged to the Beta- and Gamma-PV genera, being HPV-20 (Beta-1) the most prevalent type. Time of sun exposure and age were significantly higher in IC HPV-infected subjects. Using "hanging droplet" long PCR assay, the full length genomes of two γ-PV putative types were amplified. Novel putative types EP1 and FA91 showed 85% and 73% of sequence identity in L1 ORF to the HPV-173 (Gamma-1) and HPV-148 (Gamma-12), respectively. These findings expand our knowledge of the Papillomaviridae family.