GLOBAL GENOMIC DIVERSITY OF HUMAN PAPILLOMAVIRUS TYPE 11 (HPV11): A COMPARATIVE ANALYSIS OF 63 COMPLETE GENOME SEQUENCES

JELEN MM; CHEN Z; KOCJAN BJ; SEME K; BURT FJ; CHAN PKS; CHOUHY D; COMBRINCK CE; COUTLÉE F; ESTRADE C; FERENCZY A; FIANDER A; FRANCO EL; GARLAND SM; GIRI AA; GONZÁLEZ JV; GRÖNING A; HEIDRICH K; HIBBITTS S; HONJAK L; LUK TN; MARINIC K; MATSUKURA T; NEUMANN A; OTRBENK A; PICCONI MA; RICHARDSON H; SAGADIN M; SAHLI R; SEEDAT RY; SEVERINI A; SINCHI JL; SMAHELOVA J; TABRIZI SN; TACHEZY R; TOHME S; ULOZA V; VITKAUSKIENE A; WONG YW; ZIDOVEC LEPEJ S; BURK RD; POLJAK M

Conferencia; 29th International Papillomavirus Conference and Public Health & Clinical Workshops; 2014

Background: HPV11, one of the major causative agents of anogenital warts and laryngeal papillomas, is known to be less polymorphic in comparison to other HPV types. However, global genomic diversity of HPV11 remains unknown. Objetives: A total of 207 HPV11 isolates from the anogenital region (76 anogenital warts, 22 cervical swabs, 6 unspecified), head and neck region (50 laryngeal papillomas, 1 tracheal papilloma, 4 unspecified) and from unspedified anatomical sites (48 isolates) obtained from 13 countries (six continents) were sequenced in four regions: E5a-E5b-L1-LCR. Isolates with unique single nucleotide polymorphisms (SNPs), indels and amino acid changes were fully sequenced. Global and pairwise alignments were used for nucleotide difference calculation, maximum likelihood (RAxML) phulogenetic tree construction and lineage/sublineage assignment. Results: In total, 181/207 sequences of E5a-E5b-L1-LCR and 14 HPV11 CGs were obtained. Pairwise hererogeneity of 63 HPV11 CGs (14 from our study, 49 from GenBank) was 1.4%. Open reading frames (ORFs) differed in nucleotide diversity, from 1.3% (E7) to 4.9% (E5b). Preliminaty global phylogenetic tree of 63 HPV11 CGs placed HPV11 variants into two lineages A and B and four sublineages A1-A4. Sublineage A2 prevailed globally (55/63). Sublineage A1 consisted of 5 variants (4 European, 1 Asian), while newly assigned lineage/sublineages B, A3 and A4 consisted of one African, one North American and one European variant, respectively. A phylogenetic tree of E5a-E5b-L1-LCR concatenated sequeneces displayed identical tree topology to that of the CGs. Conclusions: Preliminary results of a comparative analysis on 63 HPV11 CGs show that HPV11 variants are not as highly conserved as previously determined and suggest the existence of new leneage/sublineages, which preliminary do not show any geographical or anatomical site of infection-related phylogenetic clustering.