INVESTIGADORES
JACOBO Patricia Veronica
congresos y reuniones científicas
Título:
Quantification and phenotypic characterization of dendritic cells from testicular draining lymph nodes of rats undergoing autoimmune orchitis
Autor/es:
V. A. GUAZZONE; M. MARDIROSIAN; P. JACOBO; S. HOLLWEGS; M. FIJAK; L. LUSTIG
Lugar:
Giessen
Reunión:
Workshop; 5th International Workshop of Molecular Andrology; 2009
Institución organizadora:
Academia Europea de Andrología
Resumen:
Experimental autoimmune orchitis (EAO) is an organ-specific model of autoimmunity characterized by an interstitial
lymphomononuclear cell infiltrate, apoptosis and sloughing of germ cells. EAO was induced in adult male Wistar
rats by active immunization with testicular homogenate and adjuvants (E group). Rats injected with saline solution
and adjuvants were used as control group (C). We previously observed a significant increase of testicular dendritic
cells (DC) in rats undergoing EAO (Rival et al., 2006). Also we showed no significant differences in the testicular
DC expression of MHC class II, CD80 and CD86 among normal (N), C and E rats. However the upregulated
expression of CCR7 in testicular DC in EAO suggested that these cells are in a ready migratory state to enter
and homing lymph node (LN) T- and B-cell areas (Rival et al., 2007). The aim of the present work was to quantify
and characterize the DC of the draining LN from the testis and the site of immunization in a model of EAO. Flow
cytometric analysis showed a significant increase in the percentage of DC (OX62+) from testicular draining LN of
rats with orchitis (9.46±1.87) compared to N (3.22±0.67) and C (4.88±1.35) groups, confirming the results obtained
by immunohistochemistry. Also an increase in the percentage of DC was observed at the end of the immunization
period (C: 1.50±0.55; E: 9.20±1.67). The percentage of DC of draining LN from the site immunization was similar in
C and E groups. No differences were detected by flow cytometry in the MHC class II, CD80 and CD86 expression
on DC from LN among N, C and E groups.These results suggest that during the development of chronic testicular
inflammation, the DC present in the testicular draining LN amplify the immune response against testicular antigens.