Perigestational alcohol consumption compromises mouse placental growth and development at term associated to altered blastocyst´s trophectodermal quality

GUALDONI GS; BARRIL C; JACOBO PV; SOBARZO CMA; CEBRAL E

Congreso; IX SLIMP MEETING VIRTUAL 2022: 9th Latin American Symposium on Maternal-Fetal Interaction and Placenta: "Placenta ephemeral: but leaving an important and lasting imprint".; 2022

Objectives: Normal placentation, critical for fetal growth and postnatalhealth, depends on trophoectodermal development during periimplantation. Gestational alcohol ingestion leads to gestational complications, early miscarriage, and placental anomalies. However, humanalcohol consumption prior to and up to the first weeks of gestation on themature placenta and early trophoblastic etiology received little attention.We aimed to analyze potential placental morphology-growth anomalies atterm and their relationship with trophoectodermal defects from blastocystafter perigestational alcohol intake up to preimplantation.Methods: treated female mice (TF) were administered with ethanol 10% indrinking water for 15 days before and up to day 4 of gestation (D4, preimplantation), or pregnancy continued without ethanol until day 18 (D18).Control females (CF) consumed water. At D4, trophoectodermal-dependent morpho-functional processes (blastocyst cavitation and expansion)(inverted phase-contrast microscopy), morphogenesis of intercellularadherent (AJ) and tight junctions (TJ) (immunofluorescence, confocal),proliferation (Tarkowski), and the mitotic/apoptosis (TUNEL) indexes ofblastocyst trophoectoderm were performed. At D18 histological (H&E) andmorphometrical (PAS, Image J) analyses were conducted in matureplacentas.Results: At D18, TF-placental weight was reduced, but fetus/placental index was increased vs CF (p