STUDY OF SPERMATOGONIA POPULATION IN INFERTILE PATIENTS WITH IMMUNE CELLS INFILTRATES

GONZÁLEZ LN; AMARILLA MS; MÉNDEZ CS; OXILIA H; FULCO MF; JACOBO PV; SOBARZO C; THEAS MS

Mar de Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

Azoospermia is linked to local testicular microenvironment deregulation,with inflammatory cells present in the 15% of testicular biopsiesof infertile patients. The aim of this study was to study the kinetics behaviorof spermatogonia (SP) population in infertile patients displayingtesticular immune cell infiltrates. Biopsies obtained from infertilepatients (Hospital. Clínicas, CABA) with obstructive and non-obstructiveazoospermia were classified according to morphologicalcriteria in: normal spermatogenesis (N, n=7), hypoespermatogenesis(Hypo, n=5) and severe hypospermatogenesis (SHypo, n=5).The number of immune cells was quantified by immunohistochemistryemploying the leukocyte common antigen marker (CD45) andthe number of SP (A dark, A pale and B) by morphological criteriain the same slides. The number of CD45 cells increased significantlyin Hypo and SHypo biopsies vs N (Mean±SEM N:22.150±2.510,Hypo:69.090±9.398, SHypo:95.180±12.54, p