UNDIFFERENTIATED SPERMATOGONIA CELL CYCLE ARREST CONRIBUTE TO THE IMPAIRMENT OF SPERMATOGENESIS IN THE TESTIS UNDER A CHRONIC INFLAMMATORY PROCESS

MÉNDEZ CS; FERREIRO E; SOBARZO C; LUSTIG L; JACOBO P; THEAS MS

CABA

Congreso; I Reunión Conjunta de Sociedades Biomédicas; 2017

Testis inflammation and infections are frequent causes of male infertilityand are associated to spermatogenic arrest. We developed anexperimental model of autoimmune orquitis (EAO) useful to understandthe mechanisms underlying spermatogenesis disruption. EAOis characterized by an interstitial lymphomonocyte cell infiltrate, moderatein the focal phase and abundant in severe EAO, higher levelsof nitric oxide (NO) and TNFα, apoptosis of post-meiotic germ cells(GC) and cell cycle arrest of pre-meiotic GC, spermatogonia (EP)and preleptotene spermatocytes. The aim of this study was analyzethe cell cycle of undifferentiated EP (CD9+) and evaluate if NO andTNFα are able to regulate cell cycle progression of pre-meiotic GC.Flow cytometric analysis showed that in focal EAO the % of EPCD9+in each phase of the cell cycle was similar to normal (N) rats (G1N:73.52±1.24, EAO:72.42±0.97; S N:11.56±0.70, EAO:10.76±0.89;G2 N:14.88±0.79, EAO:16.79±0.58). In severe EAO the % ofEPCD9+ significantly decreased in G1 and increased in G2 phasevs N rats (G1 N:72.45±1.78, EAO:32.06±6.69*; S N:11.17±1.83,EAO:10.68±3.15; G2 N:16.43±1.22, EAO:56.84±9.41*, *p