Delayed implantation site development and impaired decidual angiogenesis via VEGF-KDR system disruption during mouse gastrulation following perigestational alcohol ingestion

BARRIL CAMILA; GUALDONI GISELA S.; JACOBO PATRICIA V.; SOBARZO CRISTIAN MA.; CEBRAL ELISA

Congreso; IX SLIMP VIRTUAL MEETING; 2022

SLIMP: Sociedad Latinoamericana de Interacción Materno-Fetal y Placenta ? Latin American Society for Materno Fetal Interaction and Placenta

Perigestational alcohol consumption up to organogenesis produces embryo growth restriction and defective maternal vascularization by reduced uNKs and VEGF expression. Objective. To determine whether perigestational alcohol intake up to gastrulation produces early delayed growth of implantation sites (IS) and earlier alters decidual angiogenesis, uNK cell populations and VEGF-KDR-MMP-9 system.Methodology. Ethanol 10% was administered to female mice (TF) for 17 days before and up to days 8 and 8.5 of gestation. Free-ethanol water was administered to control females (CF). Histology (H-E, PAS), DBA-histochemistry, immunohistochemistry (IHC), immunofluorescence (IF), TUNEL and morphometry (ImageJ) were performed in D8-8.5-IS to analyse the vascular decidual tissue.Results. In TF, percentage of delayed IS (TS10a+ab) increased while advanced IS (TS10b+TS11, Theiler Scale) diminished at D8-8.5 vs CFs. With histological alterations (H-E) and low extracellular matrix deposition (PAS), TF-IS area decreased in TS10b stage and at D8 vs CF-IS. Expansion of decidual vascular lumen/IS area was reduced in D8.5-IS from TF vs CF (p