Tinkering with heme coordination in globins.

BORON I., CAPECE L., PENNACCHIETTI F., WETZLER D., BRUNO S., ABBRUZZETTI S., CHISARI L., LUQUE F., VIAPPIANI C., MARTI M., ESTRIN D. Y NADRA A.

Chascomús

Congreso; IV Latin American Meeting on Biological Inorganic Chemistry y V Workshop on Bioinorganic Chemistry; 2014

IV Latin American Meeting on Biological Inorganic Chemistry y V Workshop on Bioinorganic Chemistry

Myoglobin (Mb) is one of the most studied proteins. Its physiological function and the availability of its structure several decades ago helped to conceive Mb as the reference protein to develop and test many biophysical techniques. The iron in globins is coordinated to the 4 positions of the heme, and to a histidine residue located in the F8 position. In addition, globins exhibit a histidine residue located in the E7 position, which is close to the iron, so it could be expected to coordinate the Fe. However, typical globins such as Mb and hemoglobin are pentacoordinated. In the last decade, nevertheless, many hexacoordinated (6c) globins were discovered and several roles for hexacoordination in protein function were proposed. Among them, there is a particular interest in Neuroglobin (Ngb), which is expressed in the nervous system of vertebrates. Despite a low sequence conservation 3D structure of both proteins is highly conserved. Previous works have shown that the so called CD region is involved in heme coordination equilibrium.Our goal is to shed light on the key determinants for Heme 6c⇆5c equilibrium. For this purpose we engineered Mb and Ngb by swapping their CD region willing to exchange protein behavior. p { margin-bottom: 0cm; direction: ltr; color: rgb(0, 0, 0); line-height: 0.18cm; text-align: justify; orphans: 2; widows: 2; }p.western { font-family: "Times New Roman",serif; font-size: 12pt; }p.cjk { font-family: "Times New Roman",serif; font-size: 12pt; }p.ctl { font-family: "Times New Roman",serif; font-size: 12pt; }a:visited { color: rgb(128, 0, 128); }a.western:visited { }a.cjk:visited { }a.ctl:visited { font-family: "Times New Roman",serif; }a:link { color: rgb(0, 0, 255); }a.western:link { }a.ctl:link { font-family: "Times New Roman",serif; }p { margin-bottom: 0cm; direction: ltr; color: rgb(0, 0, 0); line-height: 0.18cm; text-align: justify; orphans: 2; widows: 2; }p.western { font-family: "Times New Roman",serif; font-size: 12pt; }p.cjk { font-family: "Times New Roman",serif; font-size: 12pt; }p.ctl { font-family: "Times New Roman",serif; font-size: 12pt; }a:visited { color: rgb(128, 0, 128); }a.western:visited { }a.cjk:visited { }a.ctl:visited { font-family: "Times New Roman",serif; }a:link { color: rgb(0, 0, 255); }a.western:link { }a.ctl:link { font-family: "Times New Roman",serif; }