6. SUSCEPTIBILY OF PROBIOTIC BOVINE VAGINAL LACTOBACILLUS TO ANTIMICROBIAL AGENTS

OTERO, M.C; SILVA DE RUIZ, C.; NADER-MACIAS, M.E.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES

Medwell Journals

Año: 2007 vol. 6 p. 132 - 138

The aim of this work was assessment of antibiotic susceptibility profile on bovine vaginal probiotic Lactobacillus. Antibiotics could affect the probiotic microorganisms colonizing when the host are exposed to therapies for other infections. We studied the sensibility of four bovine probiotic vaginal Lactobacillus strains to ampicillin, vancomycin, gentamicin, kanamycin, lincomycin, tetracyclin, chloramphenicol, erythromycin, sulphamethoxazole, metronidazole, nitrofurantoin, erythromycin, aminopenicillin+sulbactam and trimethoprim+sulphamethoxazole. The agar overlay disc diffusion and the dilution methods were performed according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The minimal inhibition concentrations (MICs) obtained showed that L. gasseri CRL1412, L. gasseri CRL1421 and L. gasseri CRL1460 have similar susceptibility profiles. L. delbruekii subsp. delbrueckii CRL1461 was the most susceptible strain. The four strains showed high MIC values for the inhibitors of nucleic acid synthesis (>500 µg/mL for sulphamethoxazole and metronidazole), which are widely used in bovine therapies. None of the lactobacilli was resistant to vancomycin. These results complete the studies for the inclusion of these strains in a probiotic product for the reconstitution of the bovine urogenital microflora with Lactobacillus. Thus prevent metritis in cows during postpartum and increase the reproductive performance in dairy herd.  Key words: Lactobacillus, susceptibility, antibiotic, probiotic, metritis.