INVESTIGADORES
OTERO Maria Claudia
artículos
Título:
SURVIVAL AND BENEFICIAL PROPERTIES OF LACTIC ACID BACTERIA FROM RANICULTURE SUBJECTED TO FREEZE-DRYING AND STORAGE
Autor/es:
MONTEL MENDOZA G; PASTERIS, S.E.; OTERO MC; NADER-MACÍAS, ME
Revista:
JOURNAL OF APPLIED BACTERIOLOGY
Editorial:
Society for Applied Microbiology
Referencias:
Año: 2014 vol. 116 p. 157 - 166
ISSN:
0021-8847
Resumen:
Aim: To evaluate the effect of freeze-drying and storage
conditions on the viability and beneficial properties of lactic acid bacteria
(LAB) for raniculture. Methods and Results: Lactococcus lactis CRL 1584, L.
lactis CRL 1827, Lactococcus garvieae CRL 1828 and Lactobacillus plantarum CRL
1606 viability under different conditions was studied. 10% lactose and 5% skim
milk + 5%lactose were excellent lyoprotectants, but 5% skim milk + 5% lactose
and whey protein concentrated (WPC) or WPC + sugars were the lower cost
lyoprotective options. The effect of temperature depended on both
lyoprotectants and storage time. Thus, for Lactococcus, skim milk, skim milk +
sucrose and WPC + sucrose were selected for lyophilization and storage at 4°C
and skim milk + lactose for 25°C. For Lact. plantarum CRL 1606, the best
lyoprotectants for lyophilization and storage at 4°C were milk + sugars and WPS
+ sucrose and, at 25°C, skim milk + sucrose. Conclusions: Lactic acid bacteria
viability after freeze-drying was strain specific and depended on the
lyoprotectant used. Highest viability was obtained when stored at 4°C, and the
beneficial properties remained stable for 18 months independently of storage
temperature. Significance and Impact of the Study: The studies reported for the
first time in this work are of primary interest to obtain dried bacteria to be
included in beneficial products for raniculture.