NON-CONVENTIONAL TECHNIQUES FOR PHYSICOCHEMICAL CHARACTERIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES

VALERIA SPRINGER; ALEJANDRO GONZÁLEZ FÁ; ALFREDO JUAN ; MARCELO PISTONESI; MARÍA SUSANA DI NEZIO

Simposio; 24thLatin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2018

Silver nanoparticles (AgNPs) have gained remarkable importance in the scientific/technological fields due to their unique optical and electronic properties, which are directly related to their intrinsic composition, sizes, shape and specific surface, among others. In this work green synthesized AgNPs, obtained by using carbohydrates as reducing and capping agents [1], were characterized by conventional techniques (TEM and DLS) and non-conventional techniques such as Surface Enhancement Raman spectroscopy (SERS), theoretical computational models based on the Density Functional Theory (DFT) and Capillary Electrophoresis (CE). Specifically, SERS and DFT were used to analyze the capping agents on the synthesized AgNPs. The experimentally obtained vibrational wavenumbers were comparable with those from the literature. Furthermore, D‐glucose and D‐gluconate adsorption geometries could be found on the silver surface according to the calculated normal modes and experimental calculations [2]. On the other hand, CE allowed to obtain valuable information about size distribution, zeta potential, electrophoretic mobility and stability of the obtained AgNPs in aqueous solution by a single electrophoretic run within a short analysis time (