cute and chronic postnatal stress alters glutamate transporter and behavioral stress response.

ODEON, MM; SALATINO, AE; ORTA, ML; ACOSTA, GB

Rosario

Congreso; XLI Reunión Científica Anual de la Sociedad Argentina de Farmacología Experimental; 2009

Sociedad Argentina de Farmacología Experimental

The purpose of this study was to evaluate the consequences of acute and chronic postnatal stress on adult brain on glutamate transporter (GluT) in vitro, evaluating the uptake of [3H]Glu by synaptosomes-enriched fractions isolated from rat cerebral frontal cortex (FC) and hippocampus (HIC) by time-course and kinetic studies. Also we determinated the dark-light transition test. In acute stress, while control rats were moved to a separated cage, stress rats were exposed to cold stress (4ºC) during 1 h. In repeated stress the rats were separated from their mothers and exposed to cold stress (4ºC) for 1 h at postnatal days during 20 days. These animals were allowed to a 30 days recovery period until adulthood. FC and HIC were dissected to study GluT and trunk blood samples were collected to determinate corticosterone levels. Acute stress results show in FC a decreased on time curse of Glu and increased of the Km and Vmax in all ages studied. Repeated chronic stress did not show change in uptake levels while kinetics parameters  were modified. The levels of corticosterone increased on acute stress and unchanged on chronic stress. In stress group we found an increase in the time spent in the light-side. These results suggest that the exposure to early stressful adverse life events affects hypothalamic-pituitary-adrenal (HPA) axis and alter the glutamatergic neurons. Supported by UBACYT B019