INVESTIGADORES
ALVAREZ Lucia Paula
artículos
Título:
Auxotrophic Mutant Of Staphylococcus Aureus Interferes With Nasal Colonization By The Wild Type
Autor/es:
BARBAGELATA M; ALVAREZ L; GORDIOLA M; TUCHSCHERR L; VON EIFF C; BECKER K; SORDELLI D; BUZZOLA F
Revista:
MICROBES AND INFECTION
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2011 vol. 13 p. 1081 - 1090
ISSN:
1286-4579
Resumen:
Staphylococcus
aureus nasal
carriage is a risk factor for infection in humans, particularly in the hospital
setting. Bacterial interference was used as an alternative strategy for the
prevention of upper respiratory, urogenital and gastrointestinal tract
infections. This study was designed to assess if the administration of a
live-attenuated aroA mutant of S. aureus is useful as a potential
approach to prevent transient staphylococcal nasal carriage by virulent
strains. We constructed an aroA mutant of S. aureus Newman strain
by homologous recombination. The auxotrophic NK41 mutant was attenuated as
determined by the increase of the LD50 after intraperitoneal
challenge. In mice, previous nasal colonization with the NK41 mutant
significantly reduced the number of CFU of S. aureus (HU-71 and Hde288)
clinical isolates and the parental Newman strain. The NK41 mutant was unable to
induce a pro-inflammatory response and to damage the invaded human respiratory
epithelial cells. Moreover, the cells previously or simultaneously infected
with the NK41 mutant were invaded by virulent strains in a significantly lower
degree than those of the control group. In conclusion, the attenuated NK41
mutant interfered with the colonization and establishment of pathogenic strains
of S. aureus, which produce severe infections.