Modulatory Role of Ovarian Function on Neuro-Immuno-Endocrine Axis Activity

PERELLÓ M,; GIOVAMBATTISTA A,; CASTROGIOVANNI D,; GAILLARD RC,; SPINEDI E

NEUROIMMUNOMODULATION.

KARGER

Año: 2011 p. 19 - 27

1021-7401

The aim of this study was to evaluate the effect of ovariectomy (OVX) on the acute phaseresponse of inflammatory stress. Ex vivo adrenocortical, peripheral mononuclear cell(PMNC) and adipocyte activities were studied in the intact and OVX mice. Endotoxemiawas mimicked by i.p. administration of bacterial lipoploysaccharide (LPS; 25 mg permouse) in sham operated (SHAM) and 21 day-OVX mice. Circulating corticosterone (B),tumor necrosis factor (TNF) α and leptin concentrations were monitored before and 30-120minutes post-LPS. Additionally, in vitro experiments were performed with isolatedcorticoadrenal cells, PMNC and omental adipocytes, from SHAM and OVX mice,incubated with specific secretagogues. The results indicate that while OVX enhancedTNFα secretion after LPS in vivo administration, it reduced corticoadrenal response andabrogated LPS-elicited leptin secretion in circulation. While the corticoadrenal sensitivityto ACTH stimulation was reduced by OVX, the LPS-induced PMNC response was notaffected. Exogenous leptin enhanced basal PMNC function, regardless of surgery. Finally,OVX drastically reduced in vitro adipocyte functionality. Our data support that the removalof ovaries modified neuroendocrine-immune-adipocyte axis function, and strongly suggestthat ovarian activity could play a pivotal role for the development of an adequate body’sdefense mechanism after injury.