Development of amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot

CASASNOVAS F.; FANTINI E.; PALAZZINI J.; GIAJ-MERLERA G.; CHULZE SN.; REYNOSO M.; TORRES A.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 p. 1782 - 1792

1364-5072

Aims: The objective of this work was to design an amplified fragment lengthpolymorphism (AFLP)-derived specific primer for the detection of Fusariumsolani aetiological agent of peanut brown root rot (PBRR) in plant materialand soil.Methods and Results: Specific primers for the detection of the pathogen weredesigned based on an amplified region using AFLPs. The banding patterns byAFLPs showed that isolates from diseased roots were clearly distinguishablefrom others members of the F. solani species complex. Many bands werespecific to F. solani PBRR, one of these fragments was selected and sequenced.Sequence obtained was used to develop specific PCR primers for theidentification of pathogen in pure culture and in plant material and soil.Primer pair FS1/FS2 amplified a single DNA product of 175 bp. Other fungalisolates occurring in soil, included F. solani non-PBRR, were not detected bythese specific primers. The assay was effective for the detection of pathogenfrom diseased root and infected soils.Conclusions: The designed primers for F. solani causing PBRR can be used ina PCR diagnostic protocol to rapidly and reliably detect and identify thispathogen.Significance and Impact of the Study: These diagnostic PCR primers will aid the detection of F. solani causing PBRR in diseased root and natural infected soils. The method developed could be a helpful tool for epidemiological studiesand to avoid the spread of this serious disease in new areas.