INVESTIGADORES
WELCHEN Elina
congresos y reuniones científicas
Título:
Characterization of two Arabidopsis OXR genes, members of the oxidation resistance eukaryotic gene family.
Autor/es:
WELCHEN, ELINA AND GONZALEZ, DANIEL
Lugar:
Rosario, Santa Fe, Argentina
Reunión:
Congreso; XIII Reunión LatinoamericanaXXVII Reunión Argentina de Fisiología Vegetal (SAFV); 2008
Resumen:
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Reactive
oxygen species (ROS) are toxic molecules produced as a consequence of aerobic
respiration. Mitochondria are the major source of ROS within the cell, and as a
result, numerous proteins have evolved to prevent or repair oxidative damage in
this organelle. The human OXR1 (oxidation resistance) gene is a member
of a conserved family of eukaryotic genes and is involved in the prevention of
oxidative DNA damage. Homologues are present in many eukaryotic organisms from
yeast to humans. OXR1 of yeast and humans are oxidative and heat stress
inducible genes whose products localize to the mitochondria. The most highly
conserved region of OXR proteins is the carboxyl-terminal half, which contains
a TLDc domain and a calcium binding EF-hand motif. We identified two genes in
Arabidopsis, named AtOXR1 (At2g05590) and AtOXR2 (At4g39870), with
homology to human the OXR1 gene. To gain insight into the mechanisms of AtOXR
gene regulation, we have analyzed the upstream regions for both genes and
observed GUS reporter expression mainly in vascular tissues of seedling roots.
Moreover, available data show high expression levels in dry seeds and in mature
pollen for both genes. Since hOXR1 can complement the peroxide
sensitivity of the yeast OXR1 mutant when localized in yeast
mitochondria, we made GFP and HA-tagged fusions to identify the cellular
localization and constructions to complement the yeast OXR1 mutant with
both Arabidopsis genes. Furthermore, in order to determine if the Arabidopsis
genes are functionally related with their homologues, we made use of the
spontaneous mutator phenotype of E. coli oxidative repair-deficient
mutants to study their ability to protect cells from oxidative DNA damage.