Hippocampal synaptic NFkappa B during inhibitory avoidance longterm memory consolidation in mice

SALLES A; BOCCIA MM; BLAKE MG; CORBI N; ROMANO A; FREUDENTHAL R

Chicago

Congreso; SFN Annual Meeting; 2015

SFN - SOCIETY FOR NEUROSCIENCE

NFkappa B is a transcription factor whose nuclear activity has been proven to be necessary for consolidation of long term memory in several species ranging from crabs to mice. This transcription factor has a wide distribution in the nervous system, with a wellreported presence in dendrites and synaptic terminals. This localization of the transcription factor, plus evidence pointing to different functions, is what gave rise to two general hypotheses for synaptic NFkappaB: (a) The transcription factor plays a role in the synapse to nucleus communication, and it is retrogradely transported from polarized localizations to regulate gene expression; (b) The transcription factor modulates the synaptic function locally. Evidence indicates that both mechanisms can operate simultaneously. Our work focuses on the mice hippocampus in an associative learning paradigm such as the inhibitory avoidance learning task. We pay special attention to the local role of the transcription factor at the synapse. We describe its activation and localization dynamics during memory consolidation in this task and also we report NFkappaB strongly bound to synaptic membranes. Furthermore we explore the role of synaptic NFkappaB in this associative learning paradigm and how its activation is independent of the aversive or appetitive nature of the stimulus.