The rol of AQP3 in amnion cells exposed to an osmotic stress

DI PAOLA M.; SIERRA M.N.; FERNANDEZ N.; ERLEJMAN A.G.; CASTRO-PARODI M.; DAMIANO A.E

Virtual

Congreso; LXV Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC) en el marco de la Reunión Conjunta de Sociedades de Biociencias; 2020

Sociedad Argenitna de Investigación Clínica

INTRODUCTION: AQPs in fetal membranes have been proposed toregulate the amniotic fluid volume. Altered expression of these proteinsmight be associated with oligo and polyhydramnios syndromes However, we recently observed that the blocking of AQP3 did not preventcell swelling in amnion cells. In addition, under osmotic stress thepattern expression of amnion AQP3 was different from other AQPs,suggesting a different role for this protein.OBJETIVE: To study the regulation of AQP3 and its role in the amnion.METHODS: Amnion-derived WISH cells were cultured in hypo (150mOsm) and hyperosmolar (400 mOsm) conditions. Levels of phosphorylatedERK (pERK), JUNK (pJNK) and p38 (p-p38) were studied.Nf-ĸB and tonEBP expressions were assessed in nuclear andcytosolic fractions. AQP3 expression was analyzed after the inhibitionof Nf-ĸB and tonEBP pathways with Sodium Salicylate and Cyclosporine-A, respectively. Cell viability was studied by MTT assay.Apoptosis was studied by TUNEL assay and Bax/Bcl-2 ratio after theinhibition of AQP3 using CuSO4 or the specific siRNA.RESULTS: pERK levels increased in hyperosmolarity and did notchange in hypoosmolarity (p